Coding

Part:BBa_K4343110

Designed by: huanyu li   Group: iGEM22_NNU-China   (2022-10-09)


PaElo16

PaElo16 encodes C16/18 elongase from Parietichytrium sp. which functions in the desaturase and elongase pathway and could convert C16:0 to C18:0.

Description

The wild-type Y. lipolytica Po1f can produce C18:0 under the catalysis of C16/18 elongase. PaElo16 encodes C16/18 elongase of 379 amino acids from Parietichytrium sp.. Heterologous expression of PaElo16 could catalyze C16:0 to C18:0 in Y. lipolytica. 

In addition, some studies have used C16/18 elongase from this source to produce high yields of omega-3 fatty acids.

Reference

Wang, F., Bi, Y., Diao, J., Lv, M., Cui, J., Chen, L., and Zhang, W., 2019. Metabolic engineering to enhance biosynthesis of both docosahexaenoic acid and odd-chain fatty acids in Schizochytrium sp. S31.Biotechnol Biofuels. 12, 141.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 48
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 126
    Illegal BsaI.rc site found at 1157


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